Autophagosome requires specific early Sec proteins for its formation and NSF/SNARE for vacuolar fusion.

@article{Ishihara2001AutophagosomeRS,
  title={Autophagosome requires specific early Sec proteins for its formation and NSF/SNARE for vacuolar fusion.},
  author={Naotada Ishihara and Maho Hamasaki and Sadaki Yokota and Kensuke Suzuki and Yoshiaki Kamada and Akio Kihara and Tamotsu Yoshimori and Takeshi Noda and Yoshinori Ohsumi},
  journal={Molecular biology of the cell},
  year={2001},
  volume={12 11},
  pages={
          3690-702
        }
}
Double membrane structure, autophagosome, is formed de novo in the process of autophagy in the yeast Saccharomyces cerevisiae, and many Apg proteins participate in this process. To further understand autophagy, we analyzed the involvement of factors engaged in the secretory pathway. First, we showed that Sec18p (N-ethylmaleimide-sensitive fusion protein, NSF) and Vti1p (soluble N-ethylmaleimide-sensitive fusion protein attachment protein, SNARE), and soluble N-ethylmaleimide-sensitive fusion… CONTINUE READING
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The Sccharomyces cerevisiae v-SNARE Vti1p is required for multiple membrane transport pathways to the vacuole

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