Autophagic sequestration of [14C]sucrose, introduced into rat hepatocytes by reversible electro-permeabilization.

@article{Gordon1982AutophagicSO,
  title={Autophagic sequestration of [14C]sucrose, introduced into rat hepatocytes by reversible electro-permeabilization.},
  author={P B Gordon and Per O. Seglen},
  journal={Experimental cell research},
  year={1982},
  volume={142 1},
  pages={1-14}
}
Isolated rat hepatocytes could be made permeable to small molecules such as [14C]sucrose (but not to proteins) by subjecting the cells to repeated electric discharges in a high-voltage field. During subsequent incubation at 37 degrees C, the permeability changes were reversed within 15 min, the electro-injected [14C]sucrose remaining trapped inside the re-sealed plasma membrane. Autophagic sequestration of [14C]sucrose, i.e., the transfer of radioactivity from cytosol to sedimentable vesicles… CONTINUE READING

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