Automation of the ELISpot assay for high-throughput detection of antigen-specific T-cell responses.

Abstract

The enzyme linked immunospot (ELISpot) assay is a fundamental tool in cellular immunology, providing both quantitative and qualitative information on cellular cytokine responses to defined antigens. It enables the comprehensive screening of patient derived peripheral blood mononuclear cells to reveal the antigenic restriction of T-cell responses and is an… (More)
DOI: 10.1016/j.jim.2009.02.007

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