Automation of PRM-dependent D3-Leu tracer enrichment in HDL to study the metabolism of apoA-I, LCAT and other apolipoproteins.

Abstract

We developed an automated quantification workflow for PRM-enabled detection of D3-Leu labeled apoA-I in high-density lipoprotein (HDL) isolated from humans. Subjects received a bolus injection of D3-Leu and blood was drawn at eight time points over three days. HDL was isolated and separated into six size fractions for subsequent proteolysis and PRM analysis… (More)
DOI: 10.1002/pmic.201600085

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Cite this paper

@article{Lee2017AutomationOP, title={Automation of PRM-dependent D3-Leu tracer enrichment in HDL to study the metabolism of apoA-I, LCAT and other apolipoproteins.}, author={Lang Ho Lee and Allison B. Andraski and Brett C Pieper and Hideyuki Higashi and Frank M. Sacks and Masanori Aikawa and Sasha Anna Singh}, journal={Proteomics}, year={2017}, volume={17 1-2} }