Automatic quantification of microtubule dynamics enables RNAi-screening of new mitotic spindle regulators.

@article{Sironi2011AutomaticQO,
  title={Automatic quantification of microtubule dynamics enables RNAi-screening of new mitotic spindle regulators.},
  author={Lucia Sironi and J{\'e}r{\^o}me Solon and Christian Conrad and Thomas U. Mayer and Damian Brunner and Jan Ellenberg},
  journal={Cytoskeleton},
  year={2011},
  volume={68 5},
  pages={266-78}
}
The genetic integrity of every organism depends on the faithful partitioning of its genome between two daughter cells in mitosis. In all eukaryotes, chromosome segregation requires the assembly of the mitotic spindle, a bipolar array of dynamic microtubules. Perturbations in microtubule dynamics affect spindle assembly and maintenance and ultimately result in aberrant cell divisions. To identify new regulators of microtubule dynamics within the hundreds of mitotic hits, reported in RNAi screens… CONTINUE READING