Automatic positioning of a microinjector in mouse ES cells and rice protoplasts.

@article{Matsuoka2006AutomaticPO,
  title={Automatic positioning of a microinjector in mouse ES cells and rice protoplasts.},
  author={Hideaki Matsuoka and Soichiro Shimoda and Yoshiyuki Miwa and Mikako Saito},
  journal={Bioelectrochemistry},
  year={2006},
  volume={69 2},
  pages={187-92}
}
A 2-channel (2C) microinjector was prepared by pulling a glass capillary with a theta-shaped cross section. One channel was used as a potential measuring electrode (MeaE) and the other was used as an electrophoretic introduction electrode (IntE). The 2C microinjector was propelled by an oil pressure manipulator driven by a pulse motor, while the MeaE output was recorded continuously. When the 2C microinjector penetrated the cell membrane of a mouse ES cell or a rice protoplast, the output… CONTINUE READING
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