Automated solid-phase extraction and two-step derivatisation for simultaneous analysis of basic illicit drugs in serum by GC/MS

@article{Weinmann2000AutomatedSE,
  title={Automated solid-phase extraction and two-step derivatisation for simultaneous analysis of basic illicit drugs in serum by GC/MS},
  author={Wolfgang Weinmann and Michael Renz and S. Vogt and Stefan Pollak},
  journal={International Journal of Legal Medicine},
  year={2000},
  volume={113},
  pages={229-235}
}
Abstract A combination of automated solid-phase extraction (SPE) and subsequent two-step derivatisation has been developed for the simultaneous analysis of basic drugs of abuse and cocaine metabolites in serum samples. Substances included in this procedure are morphine, codeine, methadone, cocaine, benzoylecgonine, methylecgonine, amphetamine, methamphetamine, MDMA, MDEA and MDA. SPE with mixed-mode cartridges (RP-C8 and cation-exchange) was fully automated with a Zymark RapidTrace SPE robot… Expand
Comprehensive automation of the solid phase extraction gas chromatographic mass spectrometric analysis (SPE-GC/MS) of opioids, cocaine, and metabolites from serum and other matrices
TLDR
A comprehensively automated method was developed from a validated, partly automated routine method proving that the analytical results are equivalent even near the limits of quantification (low ng/ml range). Expand
Determination of basic drugs of abuse in human serum by online extraction and LC–MS/MS
TLDR
A new quantitation method for the determination of drugs of abuse in serum by using online extraction coupled to liquid chromatography–mass spectrometry–MS has been developed and was used to quantify basic drugs in samples “from driving under the influence of drugs” cases. Expand
Development and validation of a solid-phase extraction gas chromatography–mass spectrometry method for the simultaneous quantification of methadone, heroin, cocaine and metabolites in sweat
TLDR
A sensitive and specific method is presented to simultaneously quantify methadone, heroin, cocaine and metabolites in sweat, offering effective monitoring of drug exposure during drug treatment, workplace and criminal justice monitoring programs. Expand
Simple and rapid analysis of four amphetamines in human whole blood and urine using liquid–liquid extraction without evaporation/derivatization and gas chromatography–mass spectrometry
We developed a simple and rapid analysis method for the determination of four amphetamines in human whole blood and urine using gas chromatography–mass spectrometry (GC–MS). During the course ofExpand
GC-MS determination of amphetamines in serum using on-line trifluoroacetylation.
TLDR
The derivatization method is of low cost since only 1 microL of MBTFA is used for each flash trifluoroacetylation, and satisfactory identification of 12 amphetamine derivatives could be obtained. Expand
Validated semiquantitative/quantitative screening of 51 drugs in whole blood as silylated derivatives by gas chromatography-selected ion monitoring mass spectrometry and gas chromatography electron capture detection.
A comprehensively validated procedure is presented for simultaneous semiquantitative/quantitative screening of 51 drugs of abuse or drugs potentially hazardous for traffic safety in serum, plasma orExpand
Online sample concentration and analysis of drugs of abuse in human urine by micelle to solvent stacking in capillary zone electrophoresis
TLDR
A sensitive and rapid CZE‐UV method was developed to determine drugs and their metabolites’ presence in human urine and provides an increase of sensitivity (39‐ to 55‐fold enhancement factor). Expand
Developing of LC/MS method with microwave assisted extraction for determination of methadone and it major metabolite (EDDP) in the blood of patients of methadone maintenance treatment.
TLDR
The aim of the study was develop fast high sensitive liquid chromatography coupled with mass spectrometry for determination of methadone and EDDP after microwave assisted extraction and exhibit a good intra and inter day precision. Expand
Screening and semiquantitative analysis of drugs and drugs of abuse in human serum samples using gas chromatography-mass spectrometry.
TLDR
The GC-MS method presented here is allowing screening, identification and semiquantification of the most commonly encountered drugs and drugs of abuse in human serum and can be successfully applied to analysis of real samples from clinical and forensic toxicology cases. Expand
Screening and confirmation of 62 drugs of abuse and metabolites in urine by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry.
TLDR
An ultra-high-performance liquid chromatography--quadrupole time-of-flight mass spectrometry method for the screening and confirmation of 62 drugs of abuse and their metabolites in urine was developed and the results were compared with the immunoassay method. Expand
...
1
2
3
...

References

SHOWING 1-10 OF 17 REFERENCES
Fast screening for drugs of abuse by solid-phase extraction combined with flow-injection ionspray-tandem mass spectrometry.
TLDR
The FIA-ionspray-MS-MS method is a fast, quantitative, sensitive, and highly specific alternative method to drug-screening by immunoassays, high-performance liquid chromatography, and GC-MS. Expand
Simultaneous determination of drugs of abuse (opiates, cocaine and amphetamine) in human hair by GC/MS and its application to a methadone treatment program.
TLDR
A correlation of 0.63 was found between administered methadone dosages and concentrations measured by hair analysis, and further investigation is needed to clarify interindividual differences. Expand
Bestimmungsgrenzen für den Nachweis von Cannabinoiden in Serum
TLDR
The limits of quantitation for cannabinoids in serum using automated solid-phase extraction (SPE) and gas chromatography-mass spectrometry (GC/MS) have been determined and the necessity of drug identification prior to quantitation is discussed in detail. Expand
Confirmation of marijuana, cocaine, morphine, codeine, amphetamine, methamphetamine, phencyclidine by GC/MS in urine following immunoassay screening.
TLDR
Rapid, reliable, sensitive, qualitative, and quantitative methods using small urine volumes for confirmation of marijuana, cocaine, benzoylecgonine, ecgonine methyl ester, morphine, codeine, amphetamine, methamphetamine, and phencyclidine were developed primarily for confirmation. Expand
Isolation of acidic, neutral, and basic drugs from whole blood using a single mixed-mode solid-phase extraction column.
TLDR
A solid-phase extraction procedure was developed for the isolation of acidic, neutral, and basic drugs from whole blood and the drugs with pKa's close to the pH of the extraction system appeared in both fractions. Expand
Extraction of benzoylecgonine (cocaine metabolite) and opiates (codeine and morphine) from urine samples using the Zymark RapidTrace.
TLDR
The Zymark RapidTr Trace automated solid-phase extraction system has been evaluated for use in the urine drug-testing laboratory and the advantages of extraction using the RapidTrace instead of solid- phase extraction with the manual vacuum manifold or liquid-liquid extraction methods are described. Expand
Forensic applications of mass spectrometry
GC/MS Analysis of Body Fluids for Drugs of Abuse (J. Cody and R.L. Foltz). Forensic Drug Testing by Mass Spectrometric Analysis of Hair (W.A. Baumgartner, C.-C. Cheng, T.D. Donahue, G.F. Hayes, V.A.Expand
Lethal monointoxication by overdosage of MDEA.
TLDR
The cause of death was a monointoxication by overdosage of MDEA and its metabolites and only trace amounts of MDMA could be found in urine and blood; no other drugs were detected. Expand
Recent applications of mass spectrometry in forensic toxicology
Abstract This review encompasses applications of mass spectrometry reported during the years 1989, 1990 and 1991 for the analysis of cannabinoids, cocaine, opiates, amphetamines, lysergic acidExpand
MIKROMETHODE ZUR SIMULTANEN QUANTIFIZIERUNG VON BENZOYLECGONIN, AMPHETAMIN, CODEIN UND MORPHIN IN BLUTSERUM MIT GC/MS
Es wurde eine GC/MS-Methode zur simultanen Quantifizierung von Amphetamin, Benzoylecgonin, Codein und Morphin aus 0,1 ml Serum entwickelt. Die Methode basiert auf einem StandardverfahrenExpand
...
1
2
...