Automated segmentation of the lamina cribrosa using Frangi's filter: a novel approach for rapid identification of tissue volume fraction and beam orientation in a trabeculated structure in the eye

@article{Campbell2015AutomatedSO,
  title={Automated segmentation of the lamina cribrosa using Frangi's filter: a novel approach for rapid identification of tissue volume fraction and beam orientation in a trabeculated structure in the eye},
  author={Ian C. Campbell and Baptiste Coudrillier and Johanne Mensah and Richard L. Abel and C. Ross Ethier},
  journal={Journal of The Royal Society Interface},
  year={2015},
  volume={12}
}
The lamina cribrosa (LC) is a tissue in the posterior eye with a complex trabecular microstructure. This tissue is of great research interest, as it is likely the initial site of retinal ganglion cell axonal damage in glaucoma. Unfortunately, the LC is difficult to access experimentally, and thus imaging techniques in tandem with image processing have emerged as powerful tools to study the microstructure and biomechanics of this tissue. Here, we present a staining approach to enhance the… 
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32 Citations
Background Citations
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Methods Citations
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32 Citations

Three-Dimensional Segmentation of the Ex-Vivo Anterior Lamina Cribrosa From Second-Harmonic Imaging Microscopy
TLDR
A new automated method for segmenting the microstructure of the anterior LC in the images obtained via multiphoton microscopy using a combination of ideas is presented.
Phase-Contrast Micro-Computed Tomography Measurements of the Intraocular Pressure-Induced Deformation of the Porcine Lamina Cribrosa
TLDR
It is concluded that PC μCT provides a novel high-resolution method for imaging the LC, and when combined with DVC, allows for full-field 3D measurement of ex vivo LC biomechanics at high spatial resolution.
Glaucoma-related Changes in the Mechanical Properties and Collagen Micro-architecture of the Human Sclera
TLDR
The observed biomechanical and microstructural changes could be the result of tissue remodeling occuring in glaucoma and are likely to alter the mechanical environment of the optic nerve head and contribute to axonal damage.
Effects of collagen microstructure and material properties on the deformation of the neural tissues of the lamina cribrosa.
Characterizing the Collagen Network Structure and Pressure-Induced Strains of the Human Lamina Cribrosa
TLDR
Feature of LC beam network microstructure that are indicative of greater collagen density and connectivity are associated with lower pressure-induced LC strain, potentially contributing to resistance to glaucomatous damage.
Collagen microstructural factors influencing optic nerve head biomechanics.
TLDR
The presence of a heterogeneous collagen fiber organization in the peripapillary sclera appears effective in limiting LC strain and is able to reduce strain levels at the scleral canal boundary: a transition zone prone to LC disinsertion, focal lamina cribrosa defects, and optic disc hemorrhages in glaucoma.
A Fully Automated 3D In-Vivo Delineation and Shape Parameterization of the Human Lamina Cribrosa in Optical Coherence Tomography
TLDR
The proposed algorithm enables automatically estimating, for the first time, the lamina cribrosa in 3D, further providing clinicians with a time-efficient discrimination tool supporting glaucoma diagnosis.
Segmentation and Analysis of Anterior Lamina Cribrosa Surface using Non Local MRF and Metropolis Hasting Algorithm
TLDR
The segmentation of anterior Lamina Cribrosa surface from the OCT image is an essential task for analysis of glaucomatous damage and the Metropolis-Hastings algorithm provides autocorrelation graph and distribution of samples from a probability distribution.
Scleral structure and biomechanics
Thin Lamina Cribrosa Beams Have Different Collagen Microstructure Than Thick Beams
TLDR
Thin beams were less wavy than their thicker counterparts, suggesting that thin beams may stiffen at lower IOP than thick beams, thus providing a similar level of structural support to the axons at physiologic IOP, despite the differences in width.
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