Automated precolumn derivatization system for analyzing physiological amino acids by liquid chromatography/mass spectrometry.

@article{Shimbo2010AutomatedPD,
  title={Automated precolumn derivatization system for analyzing physiological amino acids by liquid chromatography/mass spectrometry.},
  author={Kazutaka Shimbo and Shintaro Kubo and Yushi Harada and Takashi Oonuki and Takefumi Yokokura and Hiroo Yoshida and Michiko Amao and Mina Nakamura and Naoko Kageyama and Junko Yamazaki and Shin-ichi Ozawa and Kazuo Hirayama and Toshihiko Ando and Junkichi Miura and Hiroshi Miyano},
  journal={Biomedical chromatography : BMC},
  year={2010},
  volume={24 7},
  pages={683-91}
}
An automated method for high-throughput amino acid analysis, using precolumn derivatization high-performance liquid chromatography/electrospray mass spectrometry (HPLC/ESI-MS), was developed and evaluated. The precolumn derivatization step was performed in the reaction port of a home-built auto-sampler system. Amino acids were derivatized with 3-aminopyridyl-N-hydroxysuccinimidyl carbamate, and a 3 microm Wakosil-II 3C8-100HG column (100 x 2.1 mm i.d.) was used for separation. To achieve a 13… CONTINUE READING
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Validation of the determination of amino acids in plasma by high-performance liquid chromatography using automated pre-column derivatization with o-phthaldialdehyde

  • D Fekkes, A van Dalen, M Edelman, A. Voskuilen
  • Journal of Chromatography B: Biomedical Sciences…
  • 1995
Highly Influential
5 Excerpts

Activated carbamate reagent as derivatizing agent for amino compounds in highperformance liquid chromatography

  • N Nimura, K Iwaki, T Kinoshita, K Takeda, H. Ogura
  • Analytical Chemistry
  • 1986
Highly Influential
4 Excerpts

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