Automated polymerase chain reaction in capillary tubes with hot air.

@article{Wittwer1989AutomatedPC,
  title={Automated polymerase chain reaction in capillary tubes with hot air.},
  author={Carl T. Wittwer and G C Fillmore and David R. Hillyard},
  journal={Nucleic acids research},
  year={1989},
  volume={17 11},
  pages={
          4353-7
        }
}
We describe a simple, compact, inexpensive thermal cycler that can be used for the polymerase chain reaction. Based on heat transfer with air to samples in sealed capillary tubes, the apparatus resembles a recirculating hair dryer. The temperature is regulated via thermocouple input to a programmable set-point process controller that provides proportional output to a solid state relay controlling a heating coil. For efficient cooling after the denaturation step, the controller activates a… CONTINUE READING
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