Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris.

@article{Shah2015AutomatedPF,
  title={Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris.},
  author={Kartik A. Shah and John J. Clark and Brittany A Goods and Timothy J. Politano and Nicholas J. Mozdzierz and Ross M. Zimnisky and Rachel L. Leeson and J. Christopher Love and Kerry Routenberg Love},
  journal={Biotechnology and bioengineering},
  year={2015},
  volume={112 12},
  pages={2624-9}
}
Monoclonal antibodies (mAbs) that bind and neutralize human pathogens have great therapeutic potential. Advances in automated screening and liquid handling have resulted in the ability to discover antigen-specific antibodies either directly from human blood or from various combinatorial libraries (phage, bacteria, or yeast). There remain, however, bottlenecks in the cloning, expression and evaluation of such lead antibodies identified in primary screens that hinder high-throughput screening. As… CONTINUE READING

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