Automated microscopy for electron tomography.

  title={Automated microscopy for electron tomography.},
  author={Abraham J Koster and Huiyan Chen and John Sedat and David A. Agard},
  volume={46 1-4},
Instrumentation and methodology for the automatic collection of tomographic tilt series data for the three-dimensional reconstruction of single particles is described. The system consists of a Philips EM 430 TEM, with a Gatan 673 cooled slow-scan CCD camera and a Philips C400 microscope computer control unit attached. The procedure for data collection includes direct digital recording of the images on the CCD camera and the automatic measurement and correction of (a) image shifts resulting from… CONTINUE READING


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