Automated identification of epidermal keratinocytes in reflectance confocal microscopy.

  title={Automated identification of epidermal keratinocytes in reflectance confocal microscopy.},
  author={Daniel S. Gareau},
  journal={Journal of biomedical optics},
  volume={16 3},
Keratinocytes in skin epidermis, which have bright cytoplasmic contrast and dark nuclear contrast in reflectance confocal microscopy (RCM), were modeled with a simple error function reflectance profile: erf( ). Forty-two example keratinocytes were identified as a training set which characterized the nuclear size a = 8.6±2.8 μm and reflectance gradient b = 3.6±2.1 μm at the nuclear∕cytoplasmic boundary. These mean a and b parameters were used to create a rotationally symmetric erf( ) mask that… CONTINUE READING


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