Automated forward and reverse ratcheting of DNA in a nanopore at 5-Å precision

@inproceedings{Cherf2012AutomatedFA,
  title={Automated forward and reverse ratcheting of DNA in a nanopore at 5-{\AA} precision},
  author={Gerald M. Cherf and Kate R. Lieberman and Hytham Rashid and Christopher E. Lam and Kevin Karplus and Mark Akeson},
  year={2012}
}
An emerging DNA sequencing technique uses protein or solid-state pores to analyze individual strands as they are driven in single-file order past a nanoscale sensor1–3. However, uncontrolled electrophoresis of DNA through these nanopores is too fast for accurate base reads4. Here, we describe forward and reverse ratcheting of DNA templates through the α-hemolysin nanopore controlled by phi29 DNA polymerase without the need for active voltage control. DNA strands were ratcheted through the pore… CONTINUE READING
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