Automated feeding strategies for high-cell-density fed-batch cultivation of Pseudomonas putida KT2440

Abstract

Four automatic substrate feeding strategies were developed and investigated in this study to obtain rapid, repeatable, and reliable high cell densities of Pseudomonas putida KT2440 from glucose. Growth yield data of the key nutrients, Y X/Glucose, Y X/NH4, Y X/PO4, Y X/Mg, and Y CO2/Glucose, were determined to be 0.41, 5.44, 13.70, 236, and 0.65 g g−1… (More)
DOI: 10.1007/s00253-005-0191-7

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