Automated differential leucocyte counting: Present status and future trends

@article{Lewis2004AutomatedDL,
  title={Automated differential leucocyte counting: Present status and future trends},
  author={S. Mitchell Lewis},
  journal={Blut},
  year={2004},
  volume={43},
  pages={1-6}
}
  • S. Lewis
  • Published 1 July 1981
  • Medicine
  • Blut
ConclusionFinally, it must not be forgotten that the DLC is only one aspect of examination of the blood film. Morphological abnormalities of the erythrocytes or platelets, rouleaux, cryoglobulin deposits, even an unexpected finding of malaria parasites might point to the diagnosis and help solve an otherwise obscure problem. Indeed, perhaps the greatest value of performing a manual DLC is that it ensures that the blood film has been examined adequately to reveal morphological anomalies. It… 
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2 Citations

2 Citations

Estimation of lymphocyte percentage and number on the Coulter Counter, Model S Plus Phase II.
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Rejection by the instrument proved to be a helpful function occurring in the myeloid leukaemias, erythroblastosis, and in some cases when the lymphocytes differed from normal--for example, glandular fever and chronic lymphocytic leukaemia.
Automated cytochemical differential leucocyte count in patients hospitalized with acute bacterial infections.
TLDR
The high precision and reproducibility of the cytochemical differential count indicate that the variability noted in the disease categories studied is a biological one, being the result of the complex interaction of invading microorganisms with the host defence system.

References

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TLDR
It is to be hoped that automation, besides eliminating a tedious chore, might also improve the accuracy and precision of the data obtained.
Evaluation of the Hematrak differential leucocyte counter.
TLDR
The accuracy and precision of the instrument were found to be acceptable, within the limits imposed by counting 100 cells in a wedge smear.
Evaluation of an automated differential leukocyte counting system. II. Normal cell identification.
TLDR
The system’s identification of normal cells on 454 blood smear slides compared favorably with that of the standard manual method, although there appeared to be a statistical difference of approximately 2% between the instrument and laboratory identification of lymphocytes and monocytes.
When to do diffs: how often should differential counts be repeated?
TLDR
The proposed strategy of using WBC as an indicator of developing neutropenia or neutrophilia was sound and would have resulted in loss of information in about 1% of instances only, at a considerable saving in differential counts.
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TLDR
The Hemalog D proved to be a practical machine in a routine haematology laboratory and comparison with established techniques showed systematic differences in the total white blood cell count and percentages of lymphocytes.
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TLDR
There is an improvement in the diagnostic accuracy of subclassifying leukemias because of the use of cytochemistry and the increased precision of the differential demonostrates a linear correlation between the kinetics of different cell types not previously noted.
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TLDR
The Hemalog D system performs automated differential white cell counts utilizing principles of cytochemistry, electro-optical measurement and signal logic processing and makes the acquisition of sequential data on single patients more meaningful and diagnostically valuable.
A comparison of automated cytochemical analysis and conventional methods in the classification of acute leukaemia
Summary Thirty cases of untreated acute leukaemia were analysed by continuous flow automated cytochemistry on the Hemalog D differential counter, and the results compared with conventional…
Differential white cell counts by frequency distribution analysis of cell volumes
TLDR
The absolute neutrophil count obtained by volume analysis agreed well with values obtained by microscopy; the lymphocyte count did not give such good agreement, since the smaller number of cells counted gave rise to larger sampling errors.
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TLDR
The results obtained suggest that Fourier analysis may prove a valuable technique for the interpretation of cell volume histogram data, and its potential applications are discussed.
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