Automated determination of DNA using the fluorochrome Hoechst 33258.

  title={Automated determination of DNA using the fluorochrome Hoechst 33258.},
  author={Walter Sterzel and Philip Bedford and Gerhard Eisenbrand},
  journal={Analytical biochemistry},
  volume={147 2},
An automated method for the determination of DNA content in fractions from the alkaline filter elution assay of DNA damage has been developed. DNA-containing fractions are mixed with a fluorochrome (Hoechst 33258) and the DNA concentration is measured fluorometrically in a continuous-flow system. The lower limit of detection is 0.05 micrograms DNA/ml, and the linearity range under the conditions used is 0-8 micrograms DNA/ml. The standard deviation (n = 10) was found to be +/- 0.83%. The… CONTINUE READING

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