Automated detection and tracking of individual and clustered cell surface low density lipoprotein receptor molecules.

@article{Ghosh1994AutomatedDA,
  title={Automated detection and tracking of individual and clustered cell surface low density lipoprotein receptor molecules.},
  author={Richik N. Ghosh and Watt W. Webb},
  journal={Biophysical journal},
  year={1994},
  volume={66 5},
  pages={1301-18}
}
We have developed a technique to detect, recognize, and track each individual low density lipoprotein receptor (LDL-R) molecule and small receptor clusters on the surface of human skin fibroblasts. Molecular recognition and high precision (30 nm) simultaneous automatic tracking of all of the individual receptors in the cell surface population utilize quantitative time-lapse low light level digital video fluorescence microscopy analyzed by purpose-designed algorithms executed on an image… CONTINUE READING

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