Automated design of thousands of nonrepetitive parts for engineering stable genetic systems

@article{Hossain2020AutomatedDO,
  title={Automated design of thousands of nonrepetitive parts for engineering stable genetic systems},
  author={Ayaan Hossain and Eriberto Lopez and Sean M. Halper and Daniel P. Cetnar and Alexander C. Reis and D. Strickland and E. Klavins and H. Salis},
  journal={Nature Biotechnology},
  year={2020},
  pages={1-10}
}
Engineered genetic systems are prone to failure when their genetic parts contain repetitive sequences. Designing many nonrepetitive genetic parts with desired functionalities remains a difficult challenge with high computational complexity. To overcome this challenge, we developed the Nonrepetitive Parts Calculator to rapidly generate thousands of highly nonrepetitive genetic parts from specified design constraints, including promoters, ribosome-binding sites and terminators. As a demonstration… Expand
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