Automated cycle sequencing of PCR templates: relationships between fragment size, concentration and strand renaturation rates on sequencing efficiency.

Abstract

With the Applied Biosystems 373A automated DNA sequencer, we have systematically investigated the amounts of double-stranded PCR fragments of varying size (200, 564, and 1126 bp) required to give sequence of defined lengths, up to the maximum possible. Sequencing was performed on purified double-stranded PCR products using the dye-terminator chemistry and a… (More)

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Cite this paper

@article{Taylor1994AutomatedCS, title={Automated cycle sequencing of PCR templates: relationships between fragment size, concentration and strand renaturation rates on sequencing efficiency.}, author={G. Oladunni Taylor and Ian S. Dunn}, journal={DNA sequence : the journal of DNA sequencing and mapping}, year={1994}, volume={5 1}, pages={9-15} }