Automated colorimetric screen for apyrase inhibitors.

@article{Windsor2002AutomatedCS,
  title={Automated colorimetric screen for apyrase inhibitors.},
  author={J B Windsor and Carin Thomas and Louise Margaret Hurley and Stanley J. Roux and Alan Lloyd},
  journal={BioTechniques},
  year={2002},
  volume={33 5},
  pages={1024, 1026, 1028-30}
}
Apyrases are enzymes that efficiently hydrolyze ATP and ADP and may operate both inside and outside the cell. Although apyrases are important to a variety of cellular mechanisms and uses in industry, there are no available apyrase-specific inhibitors. Colorimetric assays based on the Fiske-Subbarow method for measuring inorganic phosphate are able to detect the release of inorganic phosphate from ATP and other nucleotides. We found that this type of assay could be automated and used to screen… CONTINUE READING