Automated colorimetric assay for T cell cytotoxicity.

@article{Parish1983AutomatedCA,
  title={Automated colorimetric assay for T cell cytotoxicity.},
  author={Christopher Richard Parish and Arno M{\"u}llbacher},
  journal={Journal of immunological methods},
  year={1983},
  volume={58 1-2},
  pages={225-37}
}
A colorimetric method has been developed for detecting the lysis of target cells by cytotoxic T lymphocytes (Tc). The method entails incubating Tc cells with thioglycollate-induced macrophage targets and estimating macrophage survival at the end of the assay by staining viable macrophages with the dye neutral red. The method is substantially more sensitive than the 51Cr release assay and can be used to detect alloreactive Tc cells and H-2-restricted Tc cells against viruses, haptens and minor-H… CONTINUE READING

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