Automated cation-exchange chromatography of peptides at a high sensitivity. (0-0.1 Absorbance range).

Abstract

A method is described where leaching of the column resin by pyridine-acetate buffers is minimal and excessive rise of the base-line of the chromatogram is avoided. The method can be used to fractionate peptide mixtures at a concentration of 2-20nmol/component. 

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