Automated Yeast Two-hybrid Screening for Nuclear Receptor-interacting Proteins*S

@article{Albers2005AutomatedYT,
  title={Automated Yeast Two-hybrid Screening for Nuclear Receptor-interacting Proteins*S},
  author={Michael Albers and Harald D. Kranz and Ingo Kober and Carmen Kaiser and Martin Klink and J{\"o}rg Suckow and Rainer Kern and Manfred Koegl},
  journal={Molecular \& Cellular Proteomics},
  year={2005},
  volume={4},
  pages={205 - 213}
}
High throughput analysis of protein-protein interactions is an important sector of hypothesis-generating research. Using an improved and automated version of the yeast two-hybrid system, we completed a large interaction screening project with a focus on nuclear receptors and their cofactors. A total of 425 independent yeast two-hybrid cDNA library screens resulted in 6425 potential interacting protein fragments involved in 1613 different interaction pairs. We show that simple statistical… Expand
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