Automated DNA sequencing and analysis of 106 kilobases from human chromosome 19q13.3

@article{MartnGallardo1992AutomatedDS,
  title={Automated DNA sequencing and analysis of 106 kilobases from human chromosome 19q13.3},
  author={Antonia Mart{\'i}n-Gallardo and W. Richard McCombie and J. D. Gocayne and Michael G. FitzGerald and Simon Wallace and B. M. B. Lee and Jane E. Lamerdin and S. Trapp and Jenny M. Kelley and Li Liu and Mark Dubnick and Leslie Johnston-Dow and Anthony R. Kerlavage and Pieter J. de Jong and Anthony Carrano and Chris A. Fields and J. Craig Venter},
  journal={Nature Genetics},
  year={1992},
  volume={1},
  pages={34-39}
}
A total of 116, 118 basepairs (bp) derived from three cosmids spanning the ERCC1 locus of human chromosome 19q13.3 have been sequenced with automated fluorescence-based sequencers and analysed by polymerase chain reaction amplification and computer methods. The assembled sequence forms two contigs totalling 105,831 bp, which contain a human fosB proto-oncogene, a gene encoding a protein phosphatase, two genes of unknown function and the previously-characterized ERCC1 DNA repair gene. This light… 
View on Nature
ncbi.nlm.nih.gov
90 Citations
Highly Influential Citations
2
Background Citations
8
Methods Citations
3

90 Citations

Citation Type

Citation Type

Expressed genes, Alu repeats and polymorphisms in cosmids sequenced from chromosome 4p16.3
TLDR
The sequences of three cosmids (90 kilobases) from the Huntington's disease region in chromosome 4p16.3 have been determined and the structure of one of the new genes, hda1–1, has been determined by characterizing cDNAs from a placental library.
Sequence and gene content in 52 kb including and centromeric to the G6PD gene in Xq28.
TLDR
In segments of unique sequence, computer-aided analysis predicted three possible genes, one of which has thus far been confirmed by the recovery of a corresponding cDNA, both by a direct hybridization method and by a PCR-based method based on a primer pair inferred from the genomic sequence.
Sequencing and analysis of genomic fragments from the NF1 locus.
TLDR
The sequence of five non-contiguous genomic fragments encompassing 14.4 kilobases from the NF1 locus have been determined by fluorescence-based automated DNA sequence analysis, which resulted in the identification of the intron/exon boundaries of five exons.
Genomic sequence comparison of the human and mouse XRCC1 DNA repair gene regions.
Long-range sequence analysis in Xq28: thirteen known and six candidate genes in 219.4 kb of high GC DNA between the RCP/GCP and G6PD loci.
TLDR
DNA comprising 219 447 bp was sequenced in nine cosmids and verified at > 99.9% precision, showing the orientation of transcription varies among the first eight genes, then runs centromeric to telomeric for the next five, and is in the opposite sense for the last six.
High-resolution physical mapping of a 250-kb region of human chromosome 11q24 by genomic sequence sampling (GSS).
An integrated metric physical map of human chromosome 19
TLDR
A metric physical map of human chromosome 19 has been generated and currently, the map consists of 51 ‘islands’ containing multiple clone types, whose size, order and relative distance are known.
Sequence and genomic organization of the human G-protein Golfα gene (GNAL) on chromosome 18p11, a susceptibility region for bipolar disorder and schizophrenia
TLDR
The sequence and genomic organization of the human Golfα (GNAL) gene were determined and knowledge of the sequence and structure provides essential information for further analysis of the GNAL locus at chromosome 18p11 which has been linked to bipolar disorder and schizophrenia.
cDNA cloning, genomic organization, and expression of the human RTN2 gene, a member of a gene family encoding reticulons.
TLDR
Results show differential expression in human tissues with a strikingly high expression of the 1.3-kb transcript in skeletal muscle, which is thought to be responsible for the association of the RTN1 and RTN2 proteins with the endoplasmic reticulum.
ERCC1: A comparative genomic perspective
TLDR
The ability to assess novel transcripts and identify candidate regulatory regions demonstrates the potential utility for a catalogue archiving comparative analyses for all genes involved in DNA repair.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 42 REFERENCES
Complementary DNA sequencing: expressed sequence tags and human genome project
TLDR
Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs), which will facilitate the tagging of most human genes in a few years at a fraction of the cost of complete genomic sequencing.
Automated DNA sequencing of the human HPRT locus.
Sequence identification of 2,375 human brain genes
TLDR
2,672 new, independent cDNA clones isolated from four human brain cDNA libraries are partially sequenced to generate 2,375 expressed sequence tags to nuclear-encoded genes, representing an approximate doubling of the number of human genes identified by DNA sequencing and may represent as many as 5% of the genes in the human genome.
Molecular characterization of the human excision repair gene ERCC-1: cDNA cloning and amino acid homology with the yeast DNA repair gene RAD10
Human genome organization: Alu, LINES, and the molecular structure of metaphase chromosome bands
The human growth hormone locus: nucleotide sequence, biology, and evolution.
Genomic characterization of the human DNA excision repair gene ERCC-1.
TLDR
Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ER CC-1 repair function.
A long-range restriction map of the human chromosome 19q13 region: close physical linkage between CKMM and the ERCC1 and ERCC2 genes.
TLDR
The physical ordering of genes in a relatively small area of chromosome 19, segment q13, containing the locus for myotonic dystrophy is reported, providing a framework for the construction of a larger physical map of the area, which will facilitate the search for the DM gene.
The distribution of interspersed repetitive DNA sequences in the human genome.
Constructing chromosome- and region-specific cosmid maps of the human genome.
TLDR
Two parallel approaches for creating an ordered cosmid library of human chromosome 19 and other selected subregions of the human genome are developed and the chemistry and data analysis tools to compare the ordering efficiencies of several cosmid restriction digest fingerprinting strategies are developed.
...
1
2
3
4
5
...