Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni(2)+-particles.

Abstract

Functional genomics and the application of high-throughput (HT) approaches to solve biological and medical questions are the main drivers behind the increasing need for HT parallel expression and purification of recombinant proteins. Automation is necessary to facilitate this complex multistep process. We describe, in detail, an HT-automated purification of… (More)
DOI: 10.1007/978-1-59745-196-3_9

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