Autolysis of psychrophilic bacteria from marine fish.

  title={Autolysis of psychrophilic bacteria from marine fish.},
  author={I Makarios-Laham and Robert E. Levin},
  journal={Applied and environmental microbiology},
  volume={49 4},
Two psychrophillic bacterial isolates of marine fish origin unable to grow at 20 degrees C or above were found to be distinguishable on the basis of autolysis at elevated temperature in various buffer systems. Isolate OP2 exhibited autolysis at 30 degrees C and above, while isolate OP7 underwent autolysis only at 35 degrees C and above. Tris buffer at pH 7.0 and 8.0 and at 35 degrees C significantly protected isolate OP2 from autolysis and failed to do so with isolate OP7. At pH 5.0, suspension… CONTINUE READING