Autolysis loop restricts the specificity of activated protein C: analysis by FRET and functional assays.

@article{Qureshi2008AutolysisLR,
  title={Autolysis loop restricts the specificity of activated protein C: analysis by FRET and functional assays.},
  author={Shabir Hussain Qureshi and Chandrashekhara Manithody and Jong-Sup Bae and Likui Yang and Alireza R Rezaie},
  journal={Biophysical chemistry},
  year={2008},
  volume={134 3},
  pages={
          239-45
        }
}
We previously demonstrated that the substitution of the autolysis loop (residues 143-154 in chymotrypsin numbering) of APC with the corresponding loop of trypsin (APC-Tryp 143-154) has no influence on the proteolytic activity of the protease toward fVa, however, this substitution increases the reactivity of APC with plasma inhibitors so that the mutant exhibits no anticoagulant activity in plasma. To further investigate the role of the autolysis loop in APC and determine whether this loop is a… CONTINUE READING