Autolysin-targeted LightCycler assay including internal process control for detection of Streptococcus pneumoniae DNA in clinical samples.

@article{Sheppard2004AutolysintargetedLA,
  title={Autolysin-targeted LightCycler assay including internal process control for detection of Streptococcus pneumoniae DNA in clinical samples.},
  author={Carmen L Sheppard and Timothy G. Harrison and Rhonwen Morris and Angela Hogan and RobertC. George},
  journal={Journal of medical microbiology},
  year={2004},
  volume={53 Pt 3},
  pages={
          189-95
        }
}
The development and clinical evaluation of a LightCycler PCR assay, including an internal process control (IPC), to detect the Streptococcus pneumoniae autolysin gene in clinical samples is reported. The assay was developed to provide a second target for use in conjunction with existing pneumolysin PCR assays to increase the reliability of non-culture PCR diagnosis of pneumococcal infection. Primers amplify a 173 bp fragment of the autolysin gene (lytA), which is detected by fluorescence… CONTINUE READING

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