Autologous bilayered self-assembled skin substitutes (SASSs) as permanent grafts: a case series of 14 severely burned patients indicating clinical effectiveness.

@article{Germain2018AutologousBS,
  title={Autologous bilayered self-assembled skin substitutes (SASSs) as permanent grafts: a case series of 14 severely burned patients indicating clinical effectiveness.},
  author={Lucie Germain and Danielle Larouche and B Nedelec and Isabelle Perreault and Louise Duranceau and Patricia Bortoluzzi and Chanel Beaudoin Cloutier and Herv{\'e} Genest and Louise Caouette‐Laberge and A Dumas and Anthony Bussi{\`e}re and Elie Boghossian and Jonathan Kanevsky and Y Leclerc and Jungwoon Lee and Minh Nguyen and Vincent Bernier and Bartha Maria Knoppers and V{\'e}ronique J. Moulin and François A Auger},
  journal={European cells \& materials},
  year={2018},
  volume={36},
  pages={
          128-141
        }
}
Split-thickness skin autografts (AGs) are the standard surgical treatment for severe burn injuries. However, the treatment of patients with substantial skin loss is limited by the availability of donor sites for skin harvesting. As an alternative to skin autografts, our research group developed autologous self-assembled skin substitutes (SASSs), allowing the replacement of both dermis and epidermis in a single surgical procedure. The aim of the study was to assess the clinical outcome of the… 

Figures and Tables from this paper

Biofabrication and preclinical evaluation of a large-sized human self-assembled skin substitute
TLDR
This study aims to produce a large-sized self-assembled skin substitute (L-SASS; 289 cm2) and evaluate its preclinical potential for skin wound coverage and found it shared similar contraction behavior on an agar surface, thickness, and epidermal differentiation in vitro.
Handheld instrument for wound-conformal delivery of skin precursor sheets improves healing in full-thickness burns.
TLDR
In porcine pre-clinical models of full-thickness burn, the one-step in situ formation of cell-containing biomaterial sheets using a handheld instrument that accommodates the topography of the wound is reported, indicating that this device could be introduced in a clinical setting improving dermal and epidermal regeneration.
Surviving an Extensive Burn Injury Using Advanced Skin Replacement Technologies.
  • Charis Kelly, D. Wallace, J. Fish
  • Medicine, Engineering
    Journal of burn care & research : official publication of the American Burn Association
  • 2021
TLDR
Two unique, skin replacement technologies were used to treat an 86% surface area flame burn in a pediatric patient and allowed for the unprecedented survival of a child following an extensive and life-threatening burn injury.
Specialized Living Wound Dressing Based on the Self-Assembly Approach of Tissue Engineering
TLDR
The proposed method allows for a 25-day production of a living TES, which retains its histological characteristics in vitro for at least two weeks, while considering production time, handling effort during the manufacturing process, and stability of the final product.
Cellular human tissue-engineered skin substitutes investigated for deep and difficult to heal injuries
TLDR
The use of different human cell populations for preclinical and clinical TESSs under research are summarized, remarking their strengths and limitations and discuss the future perspectives, which could be useful for wound healing purposes.
Advances in Skin Tissue Bioengineering and the Challenges of Clinical Translation
TLDR
Insight is provided into the challenges faced by surgeons and scientists to develop new therapies and deliver the results of translational research to improve patient care and the reasons for the few available commercial skin substitutes are elucidated.
Bioprinting and plastic compression of large pigmented and vascularized human dermo-epidermal skin substitutes by means of a new robotic platform
TLDR
A new robotic unit for 3D bioprinting of pigmented and pre-vascularized DESS using normal human skin derived fibroblasts, blood- and lymphatic endothelial cells, keratinocytes, and melanocytes is implemented, showing the feasibility of the approach and the integrity of the reconstituted capillary network in vivo after transplantation to immunodeficient rats.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 43 REFERENCES
Cultured skin substitutes reduce requirements for harvesting of skin autograft for closure of excised, full-thickness burns.
TLDR
These results demonstrate that CSS reduce requirements for donor skin harvesting for grafting of excised, full-thickness burns of greater than 50% TBSA with qualitative outcome that is comparable to meshed AG.
Initial experience with a composite autologous skin substitute.
Randomized, Paired-Site Comparison of Autologous Engineered Skin Substitutes and Split-Thickness Skin Graft for Closure of Extensive, Full-Thickness Burns
  • S. Boyce, Peggy S. Simpson, R. Kagan
  • Medicine, Engineering
    Journal of burn care & research : official publication of the American Burn Association
  • 2017
TLDR
Results indicate that autologous ESS reduce mortality and requirements for donor skin harvesting, for grafting of full-thickness burns of greater than 50% TBSA.
The 1999 clinical research award. Cultured skin substitutes combined with Integra Artificial Skin to replace native skin autograft and allograft for the closure of excised full-thickness burns.
TLDR
This approach to the closure of excised full-thickness burns is expected to reduce greatly the time to definitive closure of burn wounds and to reduce the morbidity associated with the harvesting of donor sites for split-thickenness skin autografts.
Comparative Assessment of Cultured Skin Substitutes and Native Skin Autograft for Treatment of Full‐Thickness Burns
TLDR
Results suggest that outcome of engrafted CSSs is not different from STAG and that increased incidence is related to decreased percentage of initial engraftment, and increased rates of CSSs may lead to improved outcome for closure of burn wounds.
Cultured Skin Substitutes Reduce Donor Skin Harvesting for Closure of Excised, Full-Thickness Burns
TLDR
The results suggest that acute-phase recovery of patients with extensive burns is facilitated and that complications are reduced by the use of CSS together with conventional skin grafting.
Production of a Bilayered Self-Assembled Skin Substitute Using a Tissue-Engineered Acellular Dermal Matrix.
TLDR
This model, by enabling the production of a skin substitute in a shorter time frame without compromising its intrinsic tissue properties, represents a promising addition to the currently available burn and wound treatments.
Improved Methods to Produce Tissue-Engineered Skin Substitutes Suitable for the Permanent Closure of Full-Thickness Skin Injuries
TLDR
The new approach for the production of high-quality human skin substitutes should allow an earlier autologous grafting for the treatment of severely burned patients.
Clinical application and histological properties of autologous tissue-engineered skin equivalents using an acellular dermal matrix.
TLDR
Histological and immunohistological findings indicated that the transplanted TESE had similar characteristics to normal human split-thickness skin grafts, suggesting that the TESE using ADM can be used for permanent repair of full-thickenness skin defects.
...
1
2
3
4
5
...