Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging.

@article{Mansfield2005AutofluorescenceRM,
  title={Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging.},
  author={James R Mansfield and Kirk W Gossage and Clifford C. Hoyt and Richard M. Levenson},
  journal={Journal of biomedical optics},
  year={2005},
  volume={10 4},
  pages={41207}
}
The ability to image and quantitate fluorescently labeled markers in vivo has generally been limited by autofluorescence of the tissue. Skin, in particular, has a strong autofluorescence signal, particularly when excited in the blue or green wavelengths. Fluorescence labels with emission wavelengths in the near-infrared are more amenable to deep-tissue imaging, because both scattering and autofluorescence are reduced as wavelengths are increased, but even in these spectral regions… CONTINUE READING

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