Autocatalytic degradation of proteins in extracts of a brewing strain of Saccharomyces cerevisiae. The role of endoproteinases and exopeptidases

@article{Slaughter2004AutocatalyticDO,
  title={Autocatalytic degradation of proteins in extracts of a brewing strain of Saccharomyces cerevisiae. The role of endoproteinases and exopeptidases},
  author={Juanita C Slaughter and Taiji Nomura},
  journal={Applied Microbiology and Biotechnology},
  year={2004},
  volume={37},
  pages={638-642}
}
Cells of a brewing strain of Saccharomyces cerevisiae NCYC 1109 were harvested after the end of fermentation, but before decline in viability had commenced, and extracted by passage through an Eaton press. Immediately after preparation the proteolytic enzymes PrA and CpY, but not PrB, could be detected in the extracts. After 24 h incubation at 25°C all three enzymes were found. PrA activity was unaffected by incubation whereas CpY activity increased up to threefold. Measurement of the release… CONTINUE READING
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