Autocatalytic cyclization of an excised intervening sequence RNA is a cleavage–ligation reaction

@article{Zaug1983AutocatalyticCO,
  title={Autocatalytic cyclization of an excised intervening sequence RNA is a cleavage–ligation reaction},
  author={Arthur J. Zaug and Paula J. Grabowski and Thomas R Cech},
  journal={Nature},
  year={1983},
  volume={301},
  pages={578-583}
}
The intervening sequence (IVS) of the Tetrahymena ribosomal RNA precursor is excised as a linear RNA molecule which subsequently cyclizes itself in a protein-independent reaction. Cyclization involves cleavage of the linear IVS RNA 15 nucleotides from its 5′ end and formation of a phosphodiester bond between the new 5′ phosphate and the original 3′-hydroxyl terminus of the IVS. This recombination mechanism is analogous to that by which splicing of the precursor RNA is achieved. The circular… CONTINUE READING