Autocatalytic Activation of the Furin Zymogen Requires Removal of the Emerging Enzyme's N-Terminus from the Active Site

@article{Gawlik2009AutocatalyticAO,
  title={Autocatalytic Activation of the Furin Zymogen Requires Removal of the Emerging Enzyme's N-Terminus from the Active Site},
  author={Katarzyna Gawlik and Sergey A. Shiryaev and WenHong Zhu and Khatereh Motamedchaboki and Roxane Desjardins and Robert Day and Albert G. Remacle and Boguslaw Stec and Alex Y Strongin},
  journal={PLoS ONE},
  year={2009},
  volume={4},
  pages={1111 - 1125}
}
BACKGROUND Before furin can act on protein substrates, it must go through an ordered process of activation. Similar to many other proteinases, furin is synthesized as a zymogen (profurin) which becomes active only after the autocatalytic removal of its auto-inhibitory prodomain. We hypothesized that to activate profurin its prodomain had to be removed and, in addition, the emerging enzyme's N-terminus had to be ejected from the catalytic cleft. METHODOLOGY/PRINCIPAL FINDINGS We constructed… CONTINUE READING

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