Autoantibodies to components of the mitotic apparatus

  title={Autoantibodies to components of the mitotic apparatus},
  author={Jerome B. Rattner and Gary J. Mack and Marvin J. Fritzler},
  journal={Molecular Biology Reports},
Autoantibodies directed to a variety of cellular antigens and organelles are a feature of autoimmune diseases. They have proven useful in a clinical setting to establish diagnosis, estimate prognosis, follow disease progression, alter therapy, and initiate new investigations. Cellular and molecular biologists have used autoantibodies as probes to identify molecules involved in key cellular processes. One of the most interesting sets of autoantibodies are those that target antigens within the… 

Antibodies to Mitotic Spindle Apparatus: Clinical Significance of NuMA and HsEg5 Autoantibodies

Although both anti-mitotic spindle apparatus antibodies are not associated to a defined autoimmune pathology, the presence of NuMA antibodies, mainly at high titers, may be an indication for a more extensive screening of CTD.

Autoimmunity against hNinein, a human centrosomal protein, in patients with rheumatoid arthritis and systemic lupus erythematosus.

Results showed that autoepitopes on autoantigen hNinein are restricted to the N-terminal region and that a more significant proportion of RA patients exhibited centrosome reactivity.

Prevalence of Anticentromere F Protein Autoantibodies in 347 Patients with Non‐Hodgkin's Lymphoma

It is demonstrated that a significant incidence of anti‐CENP‐F aAbs is observed, before any treatment, in some histological subgroups of NHL patients, which indicates the usefulness of anti-CenP‐P aAbs as a marker for some NHL subgroups.

Human autoantibodies as reagents in biomedical research

This review highlights the uses of autoantibodies that may have limited diagnostic or prognostic utility, but are nonetheless novel reagents in the prosecution of molecular cell biology.

Uncommon patterns of antinuclear antibodies recognizing mitotic spindle apparatus antigens and clinical associations

In a large cohort of ANA determinations, uncommon patterns were found in around 1% of cases, and the most frequent anti-MSA patterns found were NuMA and MSA-2.

Clinical significance of rare serum autoantibodies in rheumatic diseases: a systematic literature review

The main features associated to specific autoantibodies that are rarely identified in rheumatic disease are focused on, to increase the awareness and scientific knowledge on these autoantIBodies in different ethnic groups worldwide.

RHAMM is a centrosomal protein that interacts with dynein and maintains spindle pole stability.

It is demonstrated that a subset of cellular RHAMM localizes to the centrosome and functions in the maintenance of spindle integrity and provides a potential mechanism for this function in that RHAMm may cross-link centrosomal microtubules, through a direct interaction withmicrotubules and an association with dynein.

Prevalence and pattern of antinuclear autoantibodies in 347 patients with non‐Hodgkin's lymphoma

A significant incidence of ANA is demonstrated before any treatment in NHL occurrence, which seems to be higher in some histological subgroups with particular ANA, such as ANA directed against mitotic proteins or mitotic‐associated proteins.

Non-membranous granular organelle consisting of PCM-1: subcellular distribution and cell-cycle-dependent assembly/disassembly

It is shown by immunofluorescence microscopy that pericentriolar material 1 (PCM-1) granules are not only concentrated around centrioles but also scattered throughout the cytoplasm in various types of mouse cells, suggesting that this self-aggregation is regulated in a cell-cycle-dependent manner.



Two major autoantigen-antibody systems of the mitotic spindle apparatus.

NuMA protein and a 116-kd protein are the major targets of the autoimmune response in the mitotic apparatus, since most of the selected sera (based on IIF staining of theMitotic spindles and poles) recognized 1 of these 2 antigens.

The spindle kinesin-like protein HsEg5 is an autoantigen in systemic lupus erythematosus.

Autoantibodies to HsEg5 are found in a lower frequency than NuMA in sera that demonstrate the MSA pattern of staining and appear to be specifically associated with SLE.

Autoantibodies to the mitotic spindle apparatus in Mycoplasma pneumoniae disease

This work found anti-MS antibodies to be quite common in sera from patients with Mycoplasma pneumoniae, especially in those who developed cold agglutinins (71%).

NuMA protein is a human autoantigen.

Using immunoblot analysis, it is demonstrated that the autoantibodies that decorate the mitotic spindle poles are specific for the NuMA protein, which concludes that the Numa protein is a human autoantigen.

Identification of a family of human centromere proteins using autoimmune sera from patients with scleroderma

Examination of “preimmune” serum samples from a patient who progressively developed the symptoms of scleroderma CREST over a period of several years shows that these patients make antibody species recognizing at least three distinct epitopes on C ENP-B and two on CENP-C.

Anticentromere antibodies in subjects with no apparent connective tissue disease.

It is suggested that a positive result for ACA does not always indicate the presence of a connective tissue disease.

Autoantibodies to the centrosome (centriole) react with determinants present in the glycolytic enzyme enolase.

This work has studied autoantibodies from patients that react with the centrosome (centriole) region of the cell and found by immunoblotting techniques that these antibodies react with a 48-kDa protein.

Correlates between autoantibodies to nucleolar antigens and clinical features in patients with systemic sclerosis (scleroderma).

Antibodies against RNA polymerase I were associated with diffuse scleroderma of short duration, which was characterized by a high prevalence of internal organ involvement, including renal crisis, and anti-U3 RNP antibodies had ahigh prevalence in men with significantly less joint involvement, compared with ANoA-negative patients.