Autoantibodies to IA-2 in IDDM: location of major antigenic determinants.

  title={Autoantibodies to IA-2 in IDDM: location of major antigenic determinants.},
  author={B Zhang and M. S. Lan and Abner Louis Notkins},
  volume={46 1},
Thirty-three IDDM sera that immunoprecipitated full-length IA-2 were tested for reactivity with different fragments of the IA-2 molecule. The fragments were prepared by PCR amplification of IA-2 cDNA and by expression in a rabbit reticulocyte transcription/translation system. Whereas all 33 sera reacted with the intracellular domain (amino acid 604 to 979), none of the sera reacted with the extracellular domain of IA-2 (amino acid 31 to 577). Analysis of the reactivity of IDDM sera with the… CONTINUE READING


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