Auto-induction for high level production of biologically active reteplase in Escherichia coli.

@article{FathiRoudsari2018AutoinductionFH,
  title={Auto-induction for high level production of biologically active reteplase in Escherichia coli.},
  author={Mehrnoosh Fathi-Roudsari and Nader Maghsoudi and Amirhossein Maghsoudi and Sepideh Niazi and Morvarid Soleiman},
  journal={Protein expression and purification},
  year={2018},
  volume={151},
  pages={
          18-22
        }
}
Reteplase is a third generation tissue plasminogen activator (tPA) with a modified structure and prolonged half-life in comparison to native tPA. As a non-glycosylated protein, reteplase is expressed in Escherichia coli. Due to presence of several disulfide bonds, high level production of reteplase is complicated and needs extra steps for conversion to… CONTINUE READING