Authentication of oviductus ranae and its original animals using molecular marker.

  title={Authentication of oviductus ranae and its original animals using molecular marker.},
  author={Yang Xuegan and Wang Yiquan and Zhou Kaiya and Liu Zhongquan},
  journal={Biological & pharmaceutical bulletin},
  volume={25 8},
Two pairs of diagnostic primers, IHm01-L/IHm01-H and IHm02-L/IHm02-H, for distinguishing the Chinese crude drug Oviductus Ranae from its substitutes were designed based on sequences of Cyt b gene fragment of the original animals of the drug and substitutes. Total DNAs were extracted from crude drugs purchased from five drugstores in different regions, as well as from original animals of the drug, Rana chensinensis, and seven species of related ranid species. Diagnostic polymerase chain… CONTINUE READING
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