Australian lungfish (Neoceratodus forsteri: Dipnoi) have low genetic variation at allozyme and mitochondrial DNA loci: a conservation alert?

  title={Australian lungfish (Neoceratodus forsteri: Dipnoi) have low genetic variation at allozyme and mitochondrial DNA loci: a conservation alert?},
  author={Francesca D. Frentiu and Jennifer R Ovenden and Raewyn Street},
  journal={Conservation Genetics},
Genetic variation at allozyme and mitochondrialDNA loci was investigated in the Australianlungfish, Neoceratodus forsteri Krefft1870. Tissue samples for genetic analysis weretaken non-lethally from 278 individualsrepresenting two spatially distinct endemicpopulations (Mary and Burnett rivers), as wellas one population thought to be derived from ananthropogenic translocation in the 1890's(Brisbane river). Two of 24 allozyme lociresolved from muscle tissue were polymorphic.Mitochondrial DNA… 
Mitogenomic analysis of the Australian lungfish (Neoceratodus forsteri) reveals structuring of indigenous riverine populations and late Pleistocene movement between drainage basins
The results emphasise the utility of the full mtDNA molecule for capturing population structure in taxa with low genetic diversity and suggest that genetic exchange among coastal catchments may have been facilitated by riverine connections on the exposed continental shelf during the late Pleistocene.
Extremely Low Microsatellite Diversity but Distinct Population Structure in a Long-Lived Threatened Species, the Australian Lungfish Neoceratodus forsteri (Dipnoi)
Analysis of samples from the Burnett and Mary Rivers supports the hypothesis that the Mary River is the likely source of translocated populations in the Brisbane and North Pine rivers, which agrees with historical published records of a translocation event giving rise to these populations.
Conservation genetics and the Australian lungfish Neoceratodus forsteri; a spatio-temporal study of population structure
This study has shown that the common ATPase haplotype found in contemporary populations wasAlso present among specimens collected from 1912, and the high prevalence of rare alleles recorded from modern Mary populations was also recorded from formalin preserved specimens.
Phylogeography of the Australian freshwater turtle Chelodina expansa reveals complex relationships among inland and coastal bioregions
It is demonstrated that freshwater taxa commonly display genetic differentiation within a biogeographical region where no boundaries have been recognized, highlighting the need to uncover cryptic microbiogeographical regions to aid conservation of freshwater biota.
Evaluation of Genetic Variation in the Clown Knifefish, Chitala chitala, Using Allozymes, RAPD, and Microsatellites
An analysis of 38 allozyme loci did not reveal any polymorphism in the samples from any of the riverine localities; a possible explanation for this could be that the ancestors of Chitala could have faced a population reduction in prehistoric periods, as low allo enzyme variation is also reported for other species of ChITALa from south Asia.
Isolation and characterization of 21 polymorphic microsatellite loci in the iconic Australian lungfish, Neoceratodus forsteri, using the Ion Torrent next-generation sequencing platform
21 microsatellite loci were isolated and characterized in the vulnerable and iconic Australian lungfish, Neoceratodus forsteri, to assess effective population sizes and genetic structure in N. forstersi across its natural range in South East Queensland, Australia.
Monitoring age‐related trends in genomic diversity of Australian lungfish
The results suggest that long‐lived threatened species can maintain stable levels of intraspecific variability when sufficient reproductive opportunities exist over the course of a long lifespan.
Mitochondrial DNA variation in natural populations of endangered Indian Feather-Back Fish, Chitala chitala
The patterns of genetic diversity, haplotypes networks clearly indicated two distinct mitochondrial lineages and mismatch distribution strongly suggest a historical influence on the genetic structure of C. chitala populations.
Comparison of embryological development in the threatened Australian lungfish Neoceratodus forsteri from two sites in a Queensland river system
Comparison of embryological development between eggs and embryos found in unaltered reaches of the Brisbane River to eggs and embryo from a spawning event in Lake Wivenhoe, suggests that lungfish may face more problems than lack of shelter for the young.


Mitochondrial and allozyme genetics of incipient speciation in a landlocked population of Galaxias truttaceus (Pisces: Galaxiidae).
It is inferred that the lake populations of G. truttaceus have probably experienced at least one, severe, but transitory bottleneck possibly induced by natural selection for life-history characters essential for survival in the lacustrine habitat.
Strong genetic structuring in a habitat specialist, the Oxleyan Pygmy Perch Nannoperca oxleyana
It is suggested that four sites spread along 200 km of coastline were remarkably similar, sharing the same common haplotype at similar frequencies, suggesting that these four streams may have had a confluence relatively recently, possibly when sea levels were lower, 8000–10 000 BP.
Uses of molecular phylogenies for conservation
This work has suggested that within species, molecular phylogenies along with information on allele frequencies can be used to identify evolutionarily significant population units or areas and this information can potentially suggest strategies for managing declining or fragmented species, but this requires further theoretical and experimental study.
A revision of Australian Mesozoic and Cenozoic lungfish of the family Neoceratodontidae (Osteichthyes; Dipnoi), with a description of four new species
The taxonomy of the predominantly Australian fossil dipnoan genus, Neoceratodus, is revised and the Recent Australian lungfish and two fossil species are redefined and a new genus is erected to include three rare Tertiary species and one Mesozoic species.
A role for molecular genetics in biological conservation.
  • S. O’Brien
  • Biology, Environmental Science
    Proceedings of the National Academy of Sciences of the United States of America
  • 1994
The consequences of genetic depletion revealed by applying molecular population genetic analysis to four endangered mammals are reviewed, including African cheetah, lion, Florida panther, and humpback whale.
Unprecedented Low Levels of Genetic Variation and Inbreeding Depression in an Island Population of the Black‐Footed Rock‐Wallaby
Compared levels of genetic variation and fitness in island and mainland populations of the black‐footed rock‐wallaby (Petrogale lateralis) are compared, results indicate that the Barrow Island population of P. lateralis has unprecedented low levels of Genetic variation and suffers from inbreeding depression.
Karyotype and nuclear DNA content of the Australian lungfish, Neoceratodus forsteri (Ceratodidae: Dipnoi).
The karyotype of the lungfish, Neoceratodus forsteri, is described as 2n = 54, comprising 3 pairs of very large metacentrics, 1 pair of large submetacentrics, 13 pairs of smaller acrocentrics, and 10
Birth weight and neonatal survival of harbour seal pups are positively correlated with genetic variation measured by microsatellites
Mean d2 provides a better measure of individual genetic variability than heterozygosity for microsatellite data; and a convenient tool for assessing the effects of inbreeding and outbreeding in natural populations.
AFLP genotyping and fingerprinting.
A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences
  • M. Kimura
  • Biology
    Journal of Molecular Evolution
  • 2005
Some examples were worked out using reported globin sequences to show that synonymous substitutions occur at much higher rates than amino acid-altering substitutions in evolution.