Aurora kinase B regulates axonal outgrowth and regeneration in the spinal motor neurons of developing zebrafish

  title={Aurora kinase B regulates axonal outgrowth and regeneration in the spinal motor neurons of developing zebrafish},
  author={Serene S L Gwee and Rowan A W Radford and Sharron Chow and Monisha D Syal and Marco Morsch and Isabel Formella and Albert Lee and Emily K. Don and Andrew P. Badrock and Nicholas J Cole and Adrian K. West and Steve N S Cheung and Roger S. Chung},
  journal={Cellular and Molecular Life Sciences},
Aurora kinase B (AurkB) is a serine/threonine protein kinase with a well-characterised role in orchestrating cell division and cytokinesis, and is prominently expressed in healthy proliferating and cancerous cells. [] Key Result AurkB was prominently expressed within post-mitotic neurons of the zebrafish brain and spinal cord. The expression of AurkB varied during the development of the zebrafish spinal motor neurons.

High-throughput kinase inhibitor screening reveals roles for Aurora and Nuak kinases in neurite initiation and dendritic branching

High-throughput screening and analysis of Aurora and Nuak kinases revealed their functions and may contribute to the identification of therapeutics.

Rgs4 is a regulator of mTOR activity required for motoneuron axon outgrowth and neuronal development in zebrafish

Evidence is provided that Rgs4 facilitates signaling mediated by Akt, Erk and mTOR in order to drive axon outgrowth in motoneurons and regulate neuronal numbers.

Inhibition of Aurora Kinase B activity disrupts development and differentiation of salivary glands

The role of the cell cycle promoter aurora kinase B (AURKB) during development is determined, using embryonic salivary glands as a model, and inhibition of AURKB interfered with the expansion of the embryonic buds.

Functional characterization of Neurofilament Light b splicing and misbalance in zebrafish

In vivo imaging showed that neflb is crucial for proper neuronal development, and that disrupting the balance between its two isoforms specifically affects NF assembly and motor axon growth, with resulting motor deficits.

Functional Characterization of Neurofilament Light Splicing and Misbalance in Zebrafish

In vivo imaging showed that neflb is crucial for proper neuronal development, and that disrupting the balance between its two isoforms specifically affects the NF assembly and motor axon growth, with resultant motor deficits.

Programmed Variations of Cytokinesis Contribute to Morphogenesis in the C. elegans embryo

Investigation of cytokinesis during the invariant lineage of the C. elegans embryo finds that several parameters are reproducibly altered in different stages, suggesting specific regulation of these events during early divisions and morphogenesis.

Atypical function of a centrosomal module in WNT signalling drives contextual cancer cell motility

It is demonstrated that WNT signalling drives a distinct form of non-directional cell motility that requires a key centrosome module, but not microtubules or centrosomes, which is surprisingly shown to control migration after recruitment by Wnt-PCP proteins to the cell cortex, leading to actin remodelling and protrusive activity relevant to aggressive cancer motility.

Current Advances in Comprehending Dynamics of Regenerating Axons and Axon–Glia Interactions after Peripheral Nerve Injury in Zebrafish

This review summarizes the current knowledge and advances made in understanding the dynamics of the regenerative process of PNS axons and suggests that understanding the cellular and molecular mechanisms underlying the degeneration and regeneration of peripheral nerves will open new avenues in the treatment of acute nerve trauma or chronic conditions such as neurodegenerative diseases.

Salivary glands require Aurora Kinase B for regeneration after transient innate immune-mediated injury

The present study examined the initial mitogenic events marking the regenerative response of the murine submandibular gland (SMG), following innate immune-mediated injury and cellular depletion in response to poly (I:C) injury.

Investigating the Roles of Master Cell Cycle Regulators during Cytokinesis and Embryonic Development in Caenorhabditis elegans

It is suggested that separase cleaves an unknown substrate to promote the exocytosis of RAB-11 vesicles and paves the way for biochemical identification of substrates.



Activation of Aurora-A Is Essential for Neuronal Migration via Modulation of Microtubule Organization

It is reported that the mitotic kinase Aurora-A is critical for the regulation of microtubule organization during neuronal migration via an Aurora- a–NDEL1 pathway in the mouse and it is found that CDK5RAP2 is a key molecule that mediates functional interaction and is essential for centrosomal targeting of Aurora- A.

Specific distribution of overexpressed aurora B kinase in interphase normal epithelial cells

It is proposed that overexpressed aurora B targets the nuclear and cortical proteins during interphase, which may contribute to cancer development and tumor metastasis.

Transcriptional insights on the regenerative mechanics of axotomized neurons in vitro

The data reveal that neurons have the capability to fight insults by elevating biological antioxidants, regulating secondary messengers, suppressing apoptotic genes, controlling ion‐associated processes and by expressing cell cycle proteins that, in the context of neuronal injury, could potentially have functions outside their normal role in cell division.

The cdk5/p35 kinase is essential for neurite outgrowth during neuronal differentiation.

It is shown that both cdk5 and p35 proteins are present in the growth cones of developing neurons, suggesting a function of cdk4 in neuronal differentiation and a critical role in neurite outgrowth during neuronal differentiation.

The essential role of LIS1, NDEL1 and Aurora-A in polarity formation and microtubule organization during neurogensis

Physiological functions of LIS1 and NDEL1 in neurons have been ascribed for proteins fundamentally required for cell cycle progression and control and may exert other functions during neurogenesis in a direct or an indirect fashion.

Aurora kinases: novel therapy targets in cancers

The structure, localization and physiological functions of Aurora kinases in mitosis are presented, the oncogenic role of Aurora Kinases in tumorigenesis is described, and the outcomes of AKIs combination with conventional therapy are discussed.

Aurora A Is Essential for Early Embryonic Development and Tumor Suppression*

It is concluded that a balanced Aurora A level is critical for maintaining genomic stability and one needs to be fully aware of the potential side effects of anti-cancer therapy based on the use of Aurora A-specific inhibitors.

An essential role of the aPKC–Aurora A–NDEL1 pathway in neurite elongation by modulation of microtubule dynamics

A surprising role is identified in microtubule remodelling during neurite extension of an atypical protein kinase C–Aurora A–NDEL1 pathway that augments interaction with TPX2 and facilitates activation of Aurora A at the neurite hillock.

Aurora Kinase Inhibitors: Current Status and Outlook

This review will summarize the known Aurora kinase inhibitors currently in the clinic, and discuss the current and future directions.