Aurora-A and an Interacting Activator, the LIM Protein Ajuba, Are Required for Mitotic Commitment in Human Cells


Aurora family kinases contribute to regulation of mitosis. Using RNA interference in synchronized HeLa cells, we now show that Aurora-A is required for mitotic entry. We found that initial activation of Aurora-A in late G2 phase of the cell cycle is essential for recruitment of the cyclin B1-Cdk1 complex to centrosomes, where it becomes activated and commits cells to mitosis. A two-hybrid screen identified the LIM protein Ajuba as an Aurora-A binding protein. Ajuba and Aurora-A interact in mitotic cells and become phosphorylated as they do so. In vitro analyses revealed that Ajuba induces the autophosphorylation and consequent activation of Aurora-A. Depletion of Ajuba prevented activation of Aurora-A at centrosomes in late G2 phase and inhibited mitotic entry. Overall, our data suggest that Ajuba is an essential activator of Aurora-A in mitotic commitment.

DOI: 10.1016/S0092-8674(03)00642-1

Extracted Key Phrases

7 Figures and Tables

Showing 1-10 of 11 references

Culture of Japan and the " Research for the Future " program of The Japan Society for the Promotion of Roles of aurora-A kinase in mitotic entry and G2 checkpoint in mammalian cells

  • 2002


Showing 1-10 of 148 extracted citations


Citations per Year

4,264 Citations

Semantic Scholar estimates that this publication has received between 2,695 and 6,359 citations based on the available data.

See our FAQ for additional information.