Auranofin Enhances Sulforaphane-Mediated Apoptosis in Hepatocellular Carcinoma Hep3B Cells through Inactivation of the PI3K/Akt Signaling Pathway

@article{Hwangbo2020AuranofinES,
  title={Auranofin Enhances Sulforaphane-Mediated Apoptosis in Hepatocellular Carcinoma Hep3B Cells through Inactivation of the PI3K/Akt Signaling Pathway},
  author={Hyun Hwangbo and So Young Kim and Hyesook Lee and Shin Hyung Park and Su Hyun Hong and Cheol Park and Gi-Young Kim and Sun-Hee Leem and Jin Won Hyun and Jaehun Cheong and Yung-Hyun Choi},
  journal={Biomolecules \& Therapeutics},
  year={2020},
  volume={28},
  pages={443 - 455}
}
The thioredoxin (Trx) system plays critical roles in regulating intracellular redox levels and defending organisms against oxidative stress. Recent studies indicated that Trx reductase (TrxR) was overexpressed in various types of human cancer cells indicating that the Trx-TrxR system may be a potential target for anti-cancer drug development. This study investigated the synergistic effect of auranofin, a TrxR-specific inhibitor, on sulforaphane-mediated apoptotic cell death using Hep3B cells… Expand

Figures and Tables from this paper

Coptisine induces autophagic cell death through down-regulation of PI3K/Akt/mTOR signaling pathway and up-regulation of ROS-mediated mitochondrial dysfunction in hepatocellular carcinoma Hep3B cells.
TLDR
Coptisine-mediated autophagic cell death was regulated by PI3K/Akt/mTOR signaling and mitochondrial ROS production associated with mitochondrial dysfunction and these findings suggest that coptisine exerts its anti-cancer effects through induction of autophagy in HCC Hep3B cells. Expand
Novel SIRT Inhibitor, MHY2256, Induces Cell Cycle Arrest, Apoptosis, and Autophagic Cell Death in HCT116 Human Colorectal Cancer Cells
TLDR
Results suggest that MHY2256 could be a potential novel sirtuin inhibitor for the chemoprevention or treatment of colorectal cancer or both. Expand
ROS-Mediated Anti-Tumor Effect of Coptidis Rhizoma against Human Hepatocellular Carcinoma Hep3B Cells and Xenografts
  • S. Kim, Cheol Park, +10 authors Yung-Hyun Choi
  • Medicine
  • International journal of molecular sciences
  • 2021
TLDR
Analysis of the anti-cancer properties of Coptidis Rhizoma ethanol extract in HCC Hep3B cells and in a xenograft mouse model suggests that CR has anti-tumor effects that result from ROS generation, and may be a potential pharmacological intervention for HCC. Expand
The Thioredoxin Reductase Inhibitor Auranofin Suppresses Pulmonary Metastasis of Osteosarcoma, But Not Local Progression
TLDR
AUR represents a potential therapeutic drug for suppressing pulmonary metastasis of OS and represents a negative prognostic factor for metastasis and overall survival in patients with OS. Expand
Inhibition of oxidative stress induced-cytotoxicity by coptisine in V79-4 Chinese hamster lung fibroblasts through the induction of Nrf-2 mediated HO-1 expression
TLDR
Coptisine can be used as a potential treatment for oxidative stress-related lung disease and zinc protoporphyrin IX, a potent inhibitor of HO-1, attenuated the ROS scavenging and anti-apoptotic effects of coptisine. Expand
Betulinic Acid Restricts Human Bladder Cancer Cell Proliferation In Vitro by Inducing Caspase-Dependent Cell Death and Cell Cycle Arrest, and Decreasing Metastatic Potential
TLDR
This is the first study showing that BA suppresses the proliferation of human bladder cancer cells, which is due to induction of apoptosis, necrosis, and cell cycle arrest, and decrease of migration and invasion. Expand
The regulation of the TLR4/NF-κB and Nrf2/HO-1 signaling pathways is involved in the inhibition of lipopolysaccharide-induced inflammation and oxidative reactions by morroniside in RAW 264.7 macrophages.
TLDR
The findings suggest that morroniside exerts LPS-induced anti-inflammatory and antioxidant effects by targeting the TLR4/NF-κB and Nrf2/HO-1 signaling pathways in RAW 264.7 macrophages. Expand
Nargenicin A1 attenuates lipopolysaccharide-induced inflammatory and oxidative response by blocking the NF-κB signaling pathway
TLDR
It is suggested that nargenicin A1 is a potential functional agent to prevent inflammatory- and oxidative-mediated damage and ameliorates LPS-induced anti-inflammatory and antioxidant effects by downregulating the NF-κB signaling pathway. Expand
Inhibition of Lipopolysaccharide-Induced Inflammatory and Oxidative Responses by Trans-cinnamaldehyde in C2C12 Myoblasts
TLDR
The findings suggest that tCA exerts its inhibitory ability against LPS-induced inflammatory and antioxidant stress in C2C12 myoblasts by targeting the TLR4/NF-κB, which might be mediated by the NOXs and Nrf2/HO-1 pathways. Expand
Sulforaphane Impact on Reactive Oxygen Species (ROS) in Bladder Carcinoma
TLDR
Current understanding regarding the influence ofSFN on ROS and ROS-related pathways is summarized and a possible role of SFN in bladder cancer treatment is appraised. Expand
...
1
2
...

References

SHOWING 1-10 OF 56 REFERENCES
Auranofin, an inhibitor of thioredoxin reductase, induces apoptosis in hepatocellular carcinoma Hep3B cells by generation of reactive oxygen species.
TLDR
Findings indicate that auranofin inhibition of TrxR activity in Hep3B cells activates ROS- and caspase-dependent apoptotic signaling pathways and triggers cancer cell death. Expand
Morin enhances auranofin anticancer activity by up‐regulation of DR4 and DR5 and modulation of Bcl‐2 through reactive oxygen species generation in Hep3B human hepatocellular carcinoma cells
TLDR
Evidence is provided that morin can enhance the anticancer activity of AF in Hep3B human hepatocellular carcinoma cells, indicating that its combination could be an alternative treatment strategy for the hepato Cell carcinoma. Expand
The thioredoxin reductase inhibitor auranofin triggers apoptosis through a Bax/Bak-dependent process that involves peroxiredoxin 3 oxidation.
TLDR
It is concluded that auranofin induces apoptosis in cells through a Bax/Bak-dependent mechanism associated with selective disruption of mitochondrial redox homeostasis in conjunction with oxidation of Prx3. Expand
The role of protein binding in induction of apoptosis by phenethyl isothiocyanate and sulforaphane in human non-small lung cancer cells.
TLDR
Because PEITC is a stronger inducer of apoptosis than SFN, these results indicate that direct covalent binding to cellular proteins is an important early event in the inductionof apoptosis by the ITCs. Expand
Sulforaphane causes autophagy to inhibit release of cytochrome C and apoptosis in human prostate cancer cells.
TLDR
It is indicated that induction of autophagy represents a defense mechanism against sulforaphane-induced apoptosis in human prostate cancer cells, the first published report to convincingly document induction ofAutophagy by an isothiocyanate class of dietary chemopreventive agent. Expand
Inhibition of thioredoxin reductase by auranofin induces apoptosis in cisplatin-resistant human ovarian cancer cells.
TLDR
The effects of auranofin, a gold(I) compound clinically used as an antirheumatic agent, on cisplatin-sensitive (2008) and-resistant (C13*) cancer cells were studied and its action is particularly marked in C13* cells, indicating that no cross-resistance occurs. Expand
Sulforaphane decreases viability and telomerase activity in hepatocellular carcinoma Hep3B cells through the reactive oxygen species-dependent pathway.
TLDR
It is suggested that elevated intracellular reactive oxygen species (ROS) levels, due to exposure to SFN, has a critical role in abolishing since pretreatment with NAC, an antioxidant, resulted in the recovery of hTERT expression. Expand
Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells.
TLDR
The results strongly suggest that in addition to the activation of detoxifying enzymes, induction of apoptosis is also involved in the sulforaphane-associated chemoprevention of cancer. Expand
On the potential of thioredoxin reductase inhibitors for cancer therapy.
TLDR
The present knowledge on the potential of TrxR inhibitors and TrXR as anticancer drug target is summarized. Expand
The role of thioredoxin system in cancer: strategy for cancer therapy
TLDR
The combined inhibition of Trx system and GSH system in cancer therapy is highlighted and it is expected that a highly specific and selective antitumor agent with no cytotoxicity on human normal cells could be developed in the future. Expand
...
1
2
3
4
5
...