Attomolar protein detection using a magnetic bead surface coverage assay.

Abstract

We demonstrate a microfluidic method for ultra-sensitive protein detection in serum. First, 'large' (2.8 μm) antibody-functionalized magnetic beads specifically capture antigen from a serum matrix under active microfluidic mixing. Subsequently, the large beads loaded with the antigens are gently exposed to a surface pattern of fixed 'small' (1.0 μm… (More)
DOI: 10.1039/c3lc41285g

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