Attenuation of O(6)-methylguanine-DNA methyltransferase activity and mRNA levels by cisplatin and temozolomide in jurkat cells.

@article{DAtri2000AttenuationOO,
  title={Attenuation of O(6)-methylguanine-DNA methyltransferase activity and mRNA levels by cisplatin and temozolomide in jurkat cells.},
  author={Stefania D'Atri and Grazia Graziani and Pedro Miguel Lacal and V Nistic{\`o} and Sara Gilberti and Isabella Faraoni and Alastair JM Watson and Enzo Bonmassar and G. P. Margison},
  journal={The Journal of pharmacology and experimental therapeutics},
  year={2000},
  volume={294 2},
  pages={664-71}
}
The DNA repair protein O(6)-methylguanine-DNA methyltransferase (MGMT) is important in cellular resistance to certain alkylating antitumor agents such as the methylating drug temozolomide (TMZ). To provide a more rational basis for clinical combinations with another commonly used drug, cisplatin, we assessed the modulation of MGMT protein and mRNA levels in the human leukemic cell line Jurkat after treatment with these agents. Cisplatin decreased MGMT activity in a time- and dose-dependent… CONTINUE READING