Attenuation and cell culture adaptation of hepatitis A virus (HAV): a genetic analysis with HAV cDNA.

@article{Cohen1989AttenuationAC,
  title={Attenuation and cell culture adaptation of hepatitis A virus (HAV): a genetic analysis with HAV cDNA.},
  author={Jeffrey I. Cohen and Brian Rosenblum and StephenM. Feinstone and John R. Ticehurst and Robert H. Purcell},
  journal={Journal of virology},
  year={1989},
  volume={63 12},
  pages={
          5364-70
        }
}
RNA transcripts of hepatitis A virus (HAV) HM-175 cDNA from attenuated, cell culture-adapted HAV were infectious in cell culture. A full-length HAV cDNA from wild-type HAV (propagated in marmosets in vivo) was constructed. Chimeric cDNAs that contained portions of both wild-type and attenuated genomes were produced. Oligonucleotide-directed mutagenesis was used to engineer a point mutation into the VP1 gene of attenuated HAV cDNA, so that the sequence of this capsid protein would be identical… CONTINUE READING
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