Attenuated protein expression vectors for use in siRNA rescue experiments.

@article{Morita2012AttenuatedPE,
  title={Attenuated protein expression vectors for use in siRNA rescue experiments.},
  author={Eiji Morita and Jun Arii and Devin E. Christensen and J{\"o}rg Votteler and Wesley I. Sundquist},
  journal={BioTechniques},
  year={2012},
  volume={0 0},
  pages={1-5}
}
Transient transfection of small interfering RNA (siRNA) provides a powerful approach for studying cellular protein functions, particularly when the target protein can be re-expressed from an exogenous siRNA-resistant construct in order to rescue the knockdown phenotype, confirm siRNA target specificity, and support mutational analyses. Rescue experiments often fail, however, when siRNA-resistant constructs are expressed at suboptimal levels. Here, we describe an ensemble of mammalian protein… CONTINUE READING