Attenuated Shigella as a DNA Delivery Vehicle for DNA-Mediated Immunization

@article{Sizemore1995AttenuatedSA,
  title={Attenuated Shigella as a DNA Delivery Vehicle for DNA-Mediated Immunization},
  author={D R Sizemore and Arthur A. Branstrom and Jerald C. Sadoff},
  journal={Science},
  year={1995},
  volume={270},
  pages={299 - 303}
}
Direct inoculation of DNA, in the form of purified bacterial plasmids that are unable to replicate in mammalian cells but are able to direct cell synthesis of foreign proteins, is being explored as an approach to vaccine development. Here, a highly attenuated Shigella vector invaded mammalian cells and delivered such plasmids into the cytoplasm of cells, and subsequent production of functional foreign protein was measured. Because this Shigella vector was designed to deliver DNA to colonic… Expand

Topics from this paper

Attenuated bacteria as a DNA delivery vehicle for DNA-mediated immunization.
TLDR
In vivo delivery of a DNA expression plasmid to mucosal tissue results in the stimulation of immune responses against theplasmid-encoded foreign antigen. Expand
Attenuated Salmonella and Shigella as Carriers for DNA Vaccines
TLDR
This delivery system allows the administration of DNA vaccines together with associated bacterial immunostimulators directly to professional antigen presenting cells via human mucosal surfaces to achieve robust immune responses at both mucosal and systemic sites of the immunized animals. Expand
DNA vaccine delivery by attenuated intracellular bacteria.
TLDR
This work has shown that attenuated intracellular bacteria have been exploited for the introduction of DNA vaccine vectors into different cell types in vitro as well as in vivo and offer an attractive alternative to the direct inoculation of naked plasmid DNA. Expand
Bacteria as DNA vaccine carriers for genetic immunization.
TLDR
This review summarizes recent progress in bacteria-mediated delivery of plasmid DNA vaccines in the field of infectious diseases and cancer. Expand
Bacterial systems for the delivery of eukaryotic antigen expression vectors.
TLDR
Both intracellular localizations of the bacterial carriers seem to be suitable for successful delivery of DNA vaccine vectors. Expand
Immunogenicity of an HIV-1 gag DNA vaccine carried by attenuated Shigella.
TLDR
A strong boosting effect was observed in mice primed with DNA, suggesting utility of bacterial vectors in prime-boost vaccination regimens. Expand
Oral Plasmid DNA Delivery Systems for Genetic Immunisation
TLDR
It is shown that the polymer/liposome formulated DNA is able to elicit markedly enhanced transgene specific cytokine production following in vitro restimulation of splenocytes with recombinant antigen. Expand
Bacteria-mediated DNA transfer in gene therapy and vaccination
TLDR
The great potential of bacteria-mediated transfer of plasmid DNA encoding vaccine antigens and/or therapeutic molecules was demonstrated in experimental animal models of infectious diseases, tumours and gene deficiencies. Expand
Current status and future perspectives of DNA vaccine delivery by attenuated intracellular bacteria.
  • G. Dietrich
  • Biology, Medicine
  • Archivum immunologiae et therapiae experimentalis
  • 2000
TLDR
The exploitation of attenuated intracellular bacteria as DNA delivery vehicles makes the direct targeting of DNA vaccine vectors to professional APC feasible. Expand
Gram-positive and Gram-negative bacteria as carrier systems for DNA vaccines.
TLDR
It is found that several Gram-negative bacteria are capable of delivering plasmid vectors to human DC and the suitability of the Gram-positive bacterium Listeria monocytogenes as a vaccine carrier for the immunization of fish is tested. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 35 REFERENCES
Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector.
TLDR
The use of a novel suicide vector containing the pir-dependent R6K replicon and the sacB gene of Bacillus subtilis to construct an eae deletion mutant of EPEC is reported, establishing the utility of a new system for the construction of deletion mutations. Expand
A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria
TLDR
A new vector strategy for the insertion of foreign genes into the genomes of gram negative bacteria not closely related to Escherichia coli is developed, which is especially useful for site–directed transposon mutagenesis and for site-specific gene transfer in a wide variety of gramnegative organisms. Expand
Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria.
TLDR
A set of vector plasmids which greatly facilitate gene replacement and reverse genetics in many Gram-negative bacteria was constructed and it is demonstrated that they are extremely useful in eliminating long and tedious screening procedures. Expand
Alterations in the pathogenicity of Escherichia coli K-12 after transfer of plasmid and chromosomal genes from Shigella flexneri
A 140-megadalton plasmid (pWR110), which has previously been associated with virulence in Shigella flexneri, was transferred to Escherichia coli K-12. Segments of S. flexneri chromosomal materialExpand
Cholera toxin B subunit: an efficient transmucosal carrier-delivery system for induction of peripheral immunological tolerance.
TLDR
It is reported that a single oral administration of minute amounts of particulate or soluble antigen coupled to the B subunit of cholera toxin (CTB) can markedly suppress systemic immune responses in naive and in systemically immune animals. Expand
Shigella flexneri invasion plasmid antigens B and C: epitope location and characterization with monoclonal antibodies.
TLDR
Invasion plasmid antigens B and C are associated with the ability of shigellae to invade cultured mammalian cells and surface epitopes were shown to be highly conserved among different serotypes of Shigella spp. Expand
Small-animal model to measure efficacy and immunogenicity of Shigella vaccine strains
TLDR
The guinea pig model was used to test the efficacy of two vaccine candidates, and the immune response of the guinea pigs to the vaccine strains was examined. Expand
Plaque formation by virulent Shigella flexneri
An in vitro tissue culture plaque assay was developed to investigate the intracellular replication and intercellular spread of virulent shigellae. Shigella plaques were formed in HeLa cell monolayersExpand
Shigella flexneri induces apoptosis in infected macrophages
TLDR
This work reports on the mechanism of cytotoxicity used by S. flexneri to kill macrophages and demonstrates that other bacterial cytotoxic mechanisms do not lead to apoptosis, the first evidence that an invasive bacterial pathogen can induce suicide in its host cells. Expand
Use of a recombinant retrovirus to study post‐implantation cell lineage in mouse embryos.
TLDR
It is shown that a gene introduced into cells of mouse embryos by a retrovirus can serve as a heritable marker for the study of cell lineage in vivo and that several cell types have a pluripotential ancestor and that cell fate is progressively restricted as development proceeds. Expand
...
1
2
3
4
...