Attachment of biotinylated antibody to red blood cells: antigen-binding capacity of immunoerythrocytes and their susceptibility to lysis by complement.

@article{Muzykantov1994AttachmentOB,
  title={Attachment of biotinylated antibody to red blood cells: antigen-binding capacity of immunoerythrocytes and their susceptibility to lysis by complement.},
  author={Vladimir R Muzykantov and Ronald P Taylor},
  journal={Analytical biochemistry},
  year={1994},
  volume={223 1},
  pages={142-8}
}
A biotinylated monoclonal antibody (mAb) to human IgM (b-anti-IgM) has been attached to human red blood cells (RBC) by two different approaches. The first method is performed with biotinylated RBC (b-RBC) and involves stepwise binding of streptavidin (SA) to b-RBC followed by addition and binding of specific b-anti-IgM or b-IgG. b-RBC were prepared with differing input levels of biotin N-hydroxysuccinimide ester (BNHS). At moderate BNHS levels (100 microM) the resulting b-RBC (designated b4-RBC… CONTINUE READING

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