Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.

@article{Sheffield1989AttachmentOA,
  title={Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.},
  author={Val C. Sheffield and David R. Cox and Leonard S. Lerman and Richard M. Myers},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1989},
  volume={86 1},
  pages={232-6}
}
Denaturing gradient gel electrophoresis (DGGE) can be used to distinguish two DNA molecules that differ by as little as a single-base substitution. This method detects approximately 50% of all possible single-base changes in DNA fragments ranging from 50 to approximately 1000 base pairs. To increase the number of single-base changes that can be distinguished by DGGE, we used the polymerase chain reaction to attach a 40-base-pair G + C-rich sequence, designated a GC-clamp, to one end of… CONTINUE READING
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