Atresia in follicles grown after ovulation in the pig: measurement of increased apoptosis in granulosa cells and reduced follicular fluid estradiol-17 beta.

@article{Guthrie1995AtresiaIF,
  title={Atresia in follicles grown after ovulation in the pig: measurement of increased apoptosis in granulosa cells and reduced follicular fluid estradiol-17 beta.},
  author={Howard David Guthrie and Brian S. Cooper and Glenn R. Welch and A. D. Zakaria and Lawrence A. Johnson},
  journal={Biology of reproduction},
  year={1995},
  volume={52 4},
  pages={
          920-7
        }
}
The incidence of atresia in the first group of follicles grown after ovulation was investigated in the pig. At slaughter, 113 follicles 3-6 mm in diameter were dissected from the ovaries of four pregnant pigs per day on Days 5, 6, and 7 after the onset of estrus. Granulosa cells were isolated from each follicle. The percentage of granulosa cells containing sub-diploid amounts of DNA (%Ao cells), a measure of apoptosis, was determined for each follicle by DNA fluorescence flow cytometry of… CONTINUE READING

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